Journal: Cancer Management and Research

Article Title: AOC1 Contributes to Tumor Progression by Promoting the AKT and EMT Pathways in Gastric Cancer

doi: 10.2147/CMAR.S225229

Figure Lengend Snippet: AOC1 is highly expressed in gastric cancer tissues compared with normal tissues. ( A ) The red and gray boxes indicate gastric cancer and normal tissues, respectively. Data were obtained from the GEPIA website, including 408 gastric cancer samples and 211 controls. The y-axis indicated the log2-transformed gene expression levels. qPCR ( B ) and western blot ( C ) were performed to detect AOC1 expression in the tumor and paracancerous tissues of 30 patients with gastric cancerthe. *P<0.05; **P<0.01; ***P<0.001.

Article Snippet: Primary antibodies against AOC1 (Cat#16338-1-AP, 1:1000), GAPDH (Cat# 60004-1-Ig, 1:10,000), Bax (Cat# 50599-2-Ig, 1:6000), Bcl2 (Cat# 12789-1-AP, 1:1000), Caspase-9 (Cat# 10380-1-AP, 1:300), Caspase-3 (Cat# 19677-1-AP, 1:10,000), AKT (Cat# 60203-2-Ig, 1:5000), p-AKT (Cat# 66444-1-Ig, 1:2000), Cyclin D1 (Cat# 60186-1-Ig, 1:10,000), p70S6K (Cat# 66638-1-Ig, 1:3000), E-cadherin (Cat# 60335-1-Ig, 1:5000), N-cadherin (Cat# 66219-1-Ig, 1:5000) and SNAIL (Cat# 26183-1-AP, 1:1000) were purchased from ProteinTech (Rosemont, IL, USA).

Techniques: Transformation Assay, Gene Expression, Western Blot, Expressing

Journal: Cancer Management and Research

Article Title: AOC1 Contributes to Tumor Progression by Promoting the AKT and EMT Pathways in Gastric Cancer

doi: 10.2147/CMAR.S225229

Figure Lengend Snippet: AOC1 expression is effectively knocked down by siRNA transfection in human gastric cancer cells. qRT-PCR assay was used to detect the interference efficiencies of siRNAs targeting AOC1 on mRNA levels in human ( A ) AGS and ( B ) MKN45 cells. ( C ) Western blot validated the effectiveness of AOC1 knockdown on protein levels. A scrambled siRNA was used as the negative control (NC). All experiments were performed in triplicate, independently. *P<0.05.

Article Snippet: Primary antibodies against AOC1 (Cat#16338-1-AP, 1:1000), GAPDH (Cat# 60004-1-Ig, 1:10,000), Bax (Cat# 50599-2-Ig, 1:6000), Bcl2 (Cat# 12789-1-AP, 1:1000), Caspase-9 (Cat# 10380-1-AP, 1:300), Caspase-3 (Cat# 19677-1-AP, 1:10,000), AKT (Cat# 60203-2-Ig, 1:5000), p-AKT (Cat# 66444-1-Ig, 1:2000), Cyclin D1 (Cat# 60186-1-Ig, 1:10,000), p70S6K (Cat# 66638-1-Ig, 1:3000), E-cadherin (Cat# 60335-1-Ig, 1:5000), N-cadherin (Cat# 66219-1-Ig, 1:5000) and SNAIL (Cat# 26183-1-AP, 1:1000) were purchased from ProteinTech (Rosemont, IL, USA).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot, Knockdown, Negative Control

Journal: Cancer Management and Research

Article Title: AOC1 Contributes to Tumor Progression by Promoting the AKT and EMT Pathways in Gastric Cancer

doi: 10.2147/CMAR.S225229

Figure Lengend Snippet: AOC1 knockdown induces growth inhibition in human gastric cancer cells. ( A ) and ( B ) After transfection with siNC or si-AOC1, the viability of AGS and MKN45 cells was detected by using CCK-8 assay. ( C ) and ( D ) Clone formation ability of AOC1 silenced gastric cancer cells was detected. All experiments were performed in triplicate. *P<0.05.

Article Snippet: Primary antibodies against AOC1 (Cat#16338-1-AP, 1:1000), GAPDH (Cat# 60004-1-Ig, 1:10,000), Bax (Cat# 50599-2-Ig, 1:6000), Bcl2 (Cat# 12789-1-AP, 1:1000), Caspase-9 (Cat# 10380-1-AP, 1:300), Caspase-3 (Cat# 19677-1-AP, 1:10,000), AKT (Cat# 60203-2-Ig, 1:5000), p-AKT (Cat# 66444-1-Ig, 1:2000), Cyclin D1 (Cat# 60186-1-Ig, 1:10,000), p70S6K (Cat# 66638-1-Ig, 1:3000), E-cadherin (Cat# 60335-1-Ig, 1:5000), N-cadherin (Cat# 66219-1-Ig, 1:5000) and SNAIL (Cat# 26183-1-AP, 1:1000) were purchased from ProteinTech (Rosemont, IL, USA).

Techniques: Knockdown, Inhibition, Transfection, CCK-8 Assay

Journal: Cancer Management and Research

Article Title: AOC1 Contributes to Tumor Progression by Promoting the AKT and EMT Pathways in Gastric Cancer

doi: 10.2147/CMAR.S225229

Figure Lengend Snippet: Knockdown of AOC1 inhibits cell invasion and migration in human gastric cancer cells. ( A ) and ( C ) Transwell assays detecting the invasion and migration of human AGS and MKN45 cells. ( B ) and ( D ) Invaded and migrated cells in si-AOC1 group or NC group were counted. All experiments were performed for three repeated times. *P<0.05.

Article Snippet: Primary antibodies against AOC1 (Cat#16338-1-AP, 1:1000), GAPDH (Cat# 60004-1-Ig, 1:10,000), Bax (Cat# 50599-2-Ig, 1:6000), Bcl2 (Cat# 12789-1-AP, 1:1000), Caspase-9 (Cat# 10380-1-AP, 1:300), Caspase-3 (Cat# 19677-1-AP, 1:10,000), AKT (Cat# 60203-2-Ig, 1:5000), p-AKT (Cat# 66444-1-Ig, 1:2000), Cyclin D1 (Cat# 60186-1-Ig, 1:10,000), p70S6K (Cat# 66638-1-Ig, 1:3000), E-cadherin (Cat# 60335-1-Ig, 1:5000), N-cadherin (Cat# 66219-1-Ig, 1:5000) and SNAIL (Cat# 26183-1-AP, 1:1000) were purchased from ProteinTech (Rosemont, IL, USA).

Techniques: Knockdown, Migration

Journal: Cancer Management and Research

Article Title: AOC1 Contributes to Tumor Progression by Promoting the AKT and EMT Pathways in Gastric Cancer

doi: 10.2147/CMAR.S225229

Figure Lengend Snippet: Knockdown of AOC1 induces apoptosis in human gastric cancer cells. ( A ) and ( B ) The effect of AOC1 knockdown on the apoptosis of AGS cells was detected using flow cytometry. ( C ) and ( D ) The effect of AOC1 knockdown on the apoptosis of MKN45 cells was detected using flow cytometry. All experiments were performed in triplicate. *P<0.05.

Article Snippet: Primary antibodies against AOC1 (Cat#16338-1-AP, 1:1000), GAPDH (Cat# 60004-1-Ig, 1:10,000), Bax (Cat# 50599-2-Ig, 1:6000), Bcl2 (Cat# 12789-1-AP, 1:1000), Caspase-9 (Cat# 10380-1-AP, 1:300), Caspase-3 (Cat# 19677-1-AP, 1:10,000), AKT (Cat# 60203-2-Ig, 1:5000), p-AKT (Cat# 66444-1-Ig, 1:2000), Cyclin D1 (Cat# 60186-1-Ig, 1:10,000), p70S6K (Cat# 66638-1-Ig, 1:3000), E-cadherin (Cat# 60335-1-Ig, 1:5000), N-cadherin (Cat# 66219-1-Ig, 1:5000) and SNAIL (Cat# 26183-1-AP, 1:1000) were purchased from ProteinTech (Rosemont, IL, USA).

Techniques: Knockdown, Flow Cytometry

Journal: Cancer Management and Research

Article Title: AOC1 Contributes to Tumor Progression by Promoting the AKT and EMT Pathways in Gastric Cancer

doi: 10.2147/CMAR.S225229

Figure Lengend Snippet: Knockdown of AOC1 induces activation of the mitochondrial apoptosis pathway, and also inhibits the AKT signaling pathway and EMT process. ( A and B ) The key members of the mitochondrial apoptosis pathway, including Bax, Bcl2, Caspase-9, and Caspase-3, were detected by Western blot. ( C and D ) AKT signaling pathway members, including AKT, p-AKT, Cyclin D1, and p70S6K, were detected by Western blot. ( E and F ) EMT-related proteins, including E-cadherin, N-cadherin, SNAIL and Slug, were detected by Western blot. All experiments were performed in triplicate. *P<0.05.

Article Snippet: Primary antibodies against AOC1 (Cat#16338-1-AP, 1:1000), GAPDH (Cat# 60004-1-Ig, 1:10,000), Bax (Cat# 50599-2-Ig, 1:6000), Bcl2 (Cat# 12789-1-AP, 1:1000), Caspase-9 (Cat# 10380-1-AP, 1:300), Caspase-3 (Cat# 19677-1-AP, 1:10,000), AKT (Cat# 60203-2-Ig, 1:5000), p-AKT (Cat# 66444-1-Ig, 1:2000), Cyclin D1 (Cat# 60186-1-Ig, 1:10,000), p70S6K (Cat# 66638-1-Ig, 1:3000), E-cadherin (Cat# 60335-1-Ig, 1:5000), N-cadherin (Cat# 66219-1-Ig, 1:5000) and SNAIL (Cat# 26183-1-AP, 1:1000) were purchased from ProteinTech (Rosemont, IL, USA).

Techniques: Knockdown, Activation Assay, Western Blot

Journal: Cancer Management and Research

Article Title: AOC1 Contributes to Tumor Progression by Promoting the AKT and EMT Pathways in Gastric Cancer

doi: 10.2147/CMAR.S225229

Figure Lengend Snippet: IGF-1 blocked the inhibition of cell proliferation, migration and invasion caused by AOC1 knockdown. AOC1 low expressing cells were treated with IGF-1, an agonist of the AKT pathway. ( A ) CCK8 was performed to detected the proliferation of each group cells. ( B ) Transwell was performed to detected the migration and invasion of each group cells. *P<0.05 vs. NC group; #P<0.05 vs. si-AOC1 group.

Article Snippet: Primary antibodies against AOC1 (Cat#16338-1-AP, 1:1000), GAPDH (Cat# 60004-1-Ig, 1:10,000), Bax (Cat# 50599-2-Ig, 1:6000), Bcl2 (Cat# 12789-1-AP, 1:1000), Caspase-9 (Cat# 10380-1-AP, 1:300), Caspase-3 (Cat# 19677-1-AP, 1:10,000), AKT (Cat# 60203-2-Ig, 1:5000), p-AKT (Cat# 66444-1-Ig, 1:2000), Cyclin D1 (Cat# 60186-1-Ig, 1:10,000), p70S6K (Cat# 66638-1-Ig, 1:3000), E-cadherin (Cat# 60335-1-Ig, 1:5000), N-cadherin (Cat# 66219-1-Ig, 1:5000) and SNAIL (Cat# 26183-1-AP, 1:1000) were purchased from ProteinTech (Rosemont, IL, USA).

Techniques: Inhibition, Migration, Knockdown, Expressing

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: Quantitative PCR primer sequences.

Article Snippet: Huh-7 and Hep3B2.1–7 cells were transfected with 20 nM small interfering (si)RNAs targeting AOC1, the corresponding negative control (siNC), pcDNA3.1-AOC1 or the empty pcDNA3.1 vector (Guangzhou RiboBio Co., Ltd.), using Lipofectamine ® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C for 6 h. Huh-7 and Hep3B2.1–7 cells were harvested 48 h after transfection.

Techniques: Real-time Polymerase Chain Reaction, Sequencing

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: AOC1 expression levels in HCC tissues. (A) AOC1 mRNA expression in 85 HCC and adjacent-normal liver tissues. (B) Overall survival of 234 patients with HCC based on data from The Cancer Genome Atlas. ***P<0.001. AOC1, amine oxidase copper containing 1; HCC, hepatocellular carcinoma.

Article Snippet: Huh-7 and Hep3B2.1–7 cells were transfected with 20 nM small interfering (si)RNAs targeting AOC1, the corresponding negative control (siNC), pcDNA3.1-AOC1 or the empty pcDNA3.1 vector (Guangzhou RiboBio Co., Ltd.), using Lipofectamine ® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C for 6 h. Huh-7 and Hep3B2.1–7 cells were harvested 48 h after transfection.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: Association between AOC1 expression and clinical characteristics of patients with hepatocellular carcinoma.

Article Snippet: Huh-7 and Hep3B2.1–7 cells were transfected with 20 nM small interfering (si)RNAs targeting AOC1, the corresponding negative control (siNC), pcDNA3.1-AOC1 or the empty pcDNA3.1 vector (Guangzhou RiboBio Co., Ltd.), using Lipofectamine ® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C for 6 h. Huh-7 and Hep3B2.1–7 cells were harvested 48 h after transfection.

Techniques: Expressing, Virus, Infection

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: AOC1-knockdown suppresses HCC cell proliferation. (A and B) AOC1-knockdown and overexpression efficiencies were validated by reverse transcription-quantitative PCR and western blotting in Huh-7 and Hep3B2.1–7 cells. (C) Following transfection, cell viability was determined by Cell Counting Kit 8 analysis, and (D) cellular proliferation was measured using a colony formation assay. *P<0.05 vs. the siNC or Vector group. AOC1, amine oxidase copper containing 1; HCC, hepatocellular carcinoma; si, small interfering (RNA); NC, negative control.

Article Snippet: Huh-7 and Hep3B2.1–7 cells were transfected with 20 nM small interfering (si)RNAs targeting AOC1, the corresponding negative control (siNC), pcDNA3.1-AOC1 or the empty pcDNA3.1 vector (Guangzhou RiboBio Co., Ltd.), using Lipofectamine ® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C for 6 h. Huh-7 and Hep3B2.1–7 cells were harvested 48 h after transfection.

Techniques: Knockdown, Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Transfection, Cell Counting, Colony Assay, Plasmid Preparation, Small Interfering RNA, Negative Control

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: AOC1-knockdown suppresses HCC cell migration and invasiveness. Cellular migration and invasion ability were analyzed using (A) wound-healing and (B) Transwell assays, respectively. (C) Effect of AOC1 on HCC epithelial-mesenchymal transition-related protein levels was determined by western blotting. *P<0.05 vs. the siNC or Vector group. AOC1, amine oxidase copper containing 1; HCC, hepatocellular carcinoma; si, small interfering (RNA); NC, negative control.

Article Snippet: Huh-7 and Hep3B2.1–7 cells were transfected with 20 nM small interfering (si)RNAs targeting AOC1, the corresponding negative control (siNC), pcDNA3.1-AOC1 or the empty pcDNA3.1 vector (Guangzhou RiboBio Co., Ltd.), using Lipofectamine ® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C for 6 h. Huh-7 and Hep3B2.1–7 cells were harvested 48 h after transfection.

Techniques: Knockdown, Migration, Western Blot, Plasmid Preparation, Small Interfering RNA, Negative Control

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: AOC1-knockdown inhibits IL-6/JAK/STAT3 pathway activation in HCC cells. (A) Effect of AOC1-knockdown on HCC cell reactive oxygen species generation. (B) Gene set enrichment analysis results revealed that the IL-6/JAK/STAT3 pathway was enriched in AOC1-related HCC. (C) IL-6 levels in HCC cell lines were analyzed by ELISA. (D) Phosphorylation levels of JAK2 and STAT3 were analyzed by western blotting. *P<0.05 vs. siNC group. AOC1, amine oxidase copper containing 1; HCC, hepatocellular carcinoma; si, small interfering (RNA); NC, negative control.

Article Snippet: Huh-7 and Hep3B2.1–7 cells were transfected with 20 nM small interfering (si)RNAs targeting AOC1, the corresponding negative control (siNC), pcDNA3.1-AOC1 or the empty pcDNA3.1 vector (Guangzhou RiboBio Co., Ltd.), using Lipofectamine ® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C for 6 h. Huh-7 and Hep3B2.1–7 cells were harvested 48 h after transfection.

Techniques: Knockdown, Activation Assay, Enzyme-linked Immunosorbent Assay, Phospho-proteomics, Western Blot, Small Interfering RNA, Negative Control

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: AOC1-knockdown blocks the IL-6-induced proliferation, migration and invasiveness in HCC cell lines. HCC cell (A) viability, (B) proliferation, (C) migration and (D) invasiveness were analyzed following IL-6 treatment using Cell Counting Kit 8, colony formation, wound-healing and Transwell assays, respectively. *P<0.05 vs. siNC group; # P <0.05 vs. siNC + IL-6 group. AOC1, amine oxidase copper containing 1; HCC, hepatocellular carcinoma; si, small interfering (RNA); NC, negative control.

Article Snippet: Huh-7 and Hep3B2.1–7 cells were transfected with 20 nM small interfering (si)RNAs targeting AOC1, the corresponding negative control (siNC), pcDNA3.1-AOC1 or the empty pcDNA3.1 vector (Guangzhou RiboBio Co., Ltd.), using Lipofectamine ® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C for 6 h. Huh-7 and Hep3B2.1–7 cells were harvested 48 h after transfection.

Techniques: Knockdown, Migration, Cell Counting, Small Interfering RNA, Negative Control

Journal: Frontiers in Pharmacology

Article Title: Photodynamic Therapy of Novel Photosensitizer Ameliorates TNBS-Induced Ulcerative Colitis via Inhibition of AOC 1

doi: 10.3389/fphar.2021.746725

Figure Lengend Snippet: LD 4 -PDT suppressed the expression of AOC 1 . (A,B) Protein expression of AOC 1 in colon tissue and in HCoEpiC cells were determined by western blot analysis. (C) Gene expression of AOC 1 in HCoEpiC cells was examined by qRT-PCR. (D) Protein expression of AOC 1 was detected by immunofluorescence. Bars, 20 μm ** p < 0.01 , *** p < 0.001 vs control group; # p < 0.05 , ## p < 0.01 , ### p < 0.001 vs TNBS model group. Data were representative of three independent experiments, expressed as mean ± SD.

Article Snippet: Electrophoresis was performed at 150 V constant pressure for 50 min. A constant pressure of 100 V was set using the wet rotation method, and the film was transferred by ice bath for 2 h. The membrane was immersed in western blot blocking solution (232100; BD) and shaken gently at room temperature for 2 h. The following rabbit primary antibodies were diluted with TBST and prepared according to the manufacturer’s instructions; AOC 1 (16338-1-AP; Proteintech), AOC 1 (A6249; ABclonal), p-NF-κB (3033; CST), NF-κB (8242; CST), p- IκB (2859S; CST), IκB (4812; CST), IKK (2697; CST), AKT (4691; CST), p-AKT (4060; CST), p-IKK (ab178870; Abcam), IL-6 (WL02841; Wanleibio) and TNF-α (WL01581; Wanleibio).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Immunofluorescence

Journal: Frontiers in Pharmacology

Article Title: Photodynamic Therapy of Novel Photosensitizer Ameliorates TNBS-Induced Ulcerative Colitis via Inhibition of AOC 1

doi: 10.3389/fphar.2021.746725

Figure Lengend Snippet: LD 4 -PDT inhibited the expression of AOC 1 / AKT / IKK / NF-κB . (A–D) Protein expression of (A) p-AKT , (B) p-IKK, (C) p-IκB and (D) p-p65 in colon tissue were determined by western blot. (E–I) Protein expression of (E) p-AKT , (F) p-IKK , (G) p-IκB , (H) p-p65 (in nuclear), and (I) p-p65 (in cytoplasm) in HCoEpiC cells were determined by western blot ** p < 0.01 , *** p < 0.001 vs control group; # p < 0.05 , ## p < 0.01 , ### p < 0.001 vs TNBS model or LPS model group. Data are representative of three independent experiments, expressed as mean ± SD.

Article Snippet: Electrophoresis was performed at 150 V constant pressure for 50 min. A constant pressure of 100 V was set using the wet rotation method, and the film was transferred by ice bath for 2 h. The membrane was immersed in western blot blocking solution (232100; BD) and shaken gently at room temperature for 2 h. The following rabbit primary antibodies were diluted with TBST and prepared according to the manufacturer’s instructions; AOC 1 (16338-1-AP; Proteintech), AOC 1 (A6249; ABclonal), p-NF-κB (3033; CST), NF-κB (8242; CST), p- IκB (2859S; CST), IκB (4812; CST), IKK (2697; CST), AKT (4691; CST), p-AKT (4060; CST), p-IKK (ab178870; Abcam), IL-6 (WL02841; Wanleibio) and TNF-α (WL01581; Wanleibio).

Techniques: Expressing, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Photodynamic Therapy of Novel Photosensitizer Ameliorates TNBS-Induced Ulcerative Colitis via Inhibition of AOC 1

doi: 10.3389/fphar.2021.746725

Figure Lengend Snippet: LD 4 -PDT protected intestine via inhibition of AOC 1 . (A,B) LD 4 -PDT effect on TNF-α , IL-6 and IL-1 expression in AOC 1 knockdown HCoEpiC cells. (C,D) LD 4 -PDT effect on TNF-α , IL-6 and IL-1 expression in AOC 1 over-expression cells. ** p < 0.01, *** p < 0.001 vs control group; # p < 0.05 , ## p < 0.01 , ### p < 0.001 vs LPS groups. Data are representative of three independent experiments, expressed as mean ± SD.

Article Snippet: Electrophoresis was performed at 150 V constant pressure for 50 min. A constant pressure of 100 V was set using the wet rotation method, and the film was transferred by ice bath for 2 h. The membrane was immersed in western blot blocking solution (232100; BD) and shaken gently at room temperature for 2 h. The following rabbit primary antibodies were diluted with TBST and prepared according to the manufacturer’s instructions; AOC 1 (16338-1-AP; Proteintech), AOC 1 (A6249; ABclonal), p-NF-κB (3033; CST), NF-κB (8242; CST), p- IκB (2859S; CST), IκB (4812; CST), IKK (2697; CST), AKT (4691; CST), p-AKT (4060; CST), p-IKK (ab178870; Abcam), IL-6 (WL02841; Wanleibio) and TNF-α (WL01581; Wanleibio).

Techniques: Inhibition, Expressing, Over Expression

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: Quantitative PCR primer sequences.

Article Snippet: The membranes were blocked with 5% fat-free milk at 25°C for 1 h, and then incubated with primary antibodies overnight at 4°C, including AOC1 (cat. no. ab231558; Abcam), E-cadherin [cat. no. #3195; Cell Signaling Technology (CST)], N-cadherin (cat. no. #13116; CST), vimentin (cat. no. #5741; CST), JAK2 (cat. no. #3230; CST), p-JAK2 (Tyr1007/1008; cat. no. #3776; CST), STAT3 (cat. no. #12640; CST) and p-STAT3 (Tyr705; cat. no. #9145; CST) (all 1:1,000).

Techniques: Real-time Polymerase Chain Reaction, Sequencing

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: AOC1 expression levels in HCC tissues. (A) AOC1 mRNA expression in 85 HCC and adjacent-normal liver tissues. (B) Overall survival of 234 patients with HCC based on data from The Cancer Genome Atlas. ***P<0.001. AOC1, amine oxidase copper containing 1; HCC, hepatocellular carcinoma.

Article Snippet: The membranes were blocked with 5% fat-free milk at 25°C for 1 h, and then incubated with primary antibodies overnight at 4°C, including AOC1 (cat. no. ab231558; Abcam), E-cadherin [cat. no. #3195; Cell Signaling Technology (CST)], N-cadherin (cat. no. #13116; CST), vimentin (cat. no. #5741; CST), JAK2 (cat. no. #3230; CST), p-JAK2 (Tyr1007/1008; cat. no. #3776; CST), STAT3 (cat. no. #12640; CST) and p-STAT3 (Tyr705; cat. no. #9145; CST) (all 1:1,000).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: Association between AOC1 expression and clinical characteristics of patients with hepatocellular carcinoma.

Article Snippet: The membranes were blocked with 5% fat-free milk at 25°C for 1 h, and then incubated with primary antibodies overnight at 4°C, including AOC1 (cat. no. ab231558; Abcam), E-cadherin [cat. no. #3195; Cell Signaling Technology (CST)], N-cadherin (cat. no. #13116; CST), vimentin (cat. no. #5741; CST), JAK2 (cat. no. #3230; CST), p-JAK2 (Tyr1007/1008; cat. no. #3776; CST), STAT3 (cat. no. #12640; CST) and p-STAT3 (Tyr705; cat. no. #9145; CST) (all 1:1,000).

Techniques: Expressing, Infection

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: AOC1-knockdown suppresses HCC cell proliferation. (A and B) AOC1-knockdown and overexpression efficiencies were validated by reverse transcription-quantitative PCR and western blotting in Huh-7 and Hep3B2.1–7 cells. (C) Following transfection, cell viability was determined by Cell Counting Kit 8 analysis, and (D) cellular proliferation was measured using a colony formation assay. *P<0.05 vs. the siNC or Vector group. AOC1, amine oxidase copper containing 1; HCC, hepatocellular carcinoma; si, small interfering (RNA); NC, negative control.

Article Snippet: The membranes were blocked with 5% fat-free milk at 25°C for 1 h, and then incubated with primary antibodies overnight at 4°C, including AOC1 (cat. no. ab231558; Abcam), E-cadherin [cat. no. #3195; Cell Signaling Technology (CST)], N-cadherin (cat. no. #13116; CST), vimentin (cat. no. #5741; CST), JAK2 (cat. no. #3230; CST), p-JAK2 (Tyr1007/1008; cat. no. #3776; CST), STAT3 (cat. no. #12640; CST) and p-STAT3 (Tyr705; cat. no. #9145; CST) (all 1:1,000).

Techniques: Over Expression, Real-time Polymerase Chain Reaction, Western Blot, Transfection, Cell Counting, Colony Assay, Plasmid Preparation, Small Interfering RNA, Negative Control

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: AOC1-knockdown suppresses HCC cell migration and invasiveness. Cellular migration and invasion ability were analyzed using (A) wound-healing and (B) Transwell assays, respectively. (C) Effect of AOC1 on HCC epithelial-mesenchymal transition-related protein levels was determined by western blotting. *P<0.05 vs. the siNC or Vector group. AOC1, amine oxidase copper containing 1; HCC, hepatocellular carcinoma; si, small interfering (RNA); NC, negative control.

Article Snippet: The membranes were blocked with 5% fat-free milk at 25°C for 1 h, and then incubated with primary antibodies overnight at 4°C, including AOC1 (cat. no. ab231558; Abcam), E-cadherin [cat. no. #3195; Cell Signaling Technology (CST)], N-cadherin (cat. no. #13116; CST), vimentin (cat. no. #5741; CST), JAK2 (cat. no. #3230; CST), p-JAK2 (Tyr1007/1008; cat. no. #3776; CST), STAT3 (cat. no. #12640; CST) and p-STAT3 (Tyr705; cat. no. #9145; CST) (all 1:1,000).

Techniques: Migration, Western Blot, Plasmid Preparation, Small Interfering RNA, Negative Control

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: AOC1-knockdown inhibits IL-6/JAK/STAT3 pathway activation in HCC cells. (A) Effect of AOC1-knockdown on HCC cell reactive oxygen species generation. (B) Gene set enrichment analysis results revealed that the IL-6/JAK/STAT3 pathway was enriched in AOC1-related HCC. (C) IL-6 levels in HCC cell lines were analyzed by ELISA. (D) Phosphorylation levels of JAK2 and STAT3 were analyzed by western blotting. *P<0.05 vs. siNC group. AOC1, amine oxidase copper containing 1; HCC, hepatocellular carcinoma; si, small interfering (RNA); NC, negative control.

Article Snippet: The membranes were blocked with 5% fat-free milk at 25°C for 1 h, and then incubated with primary antibodies overnight at 4°C, including AOC1 (cat. no. ab231558; Abcam), E-cadherin [cat. no. #3195; Cell Signaling Technology (CST)], N-cadherin (cat. no. #13116; CST), vimentin (cat. no. #5741; CST), JAK2 (cat. no. #3230; CST), p-JAK2 (Tyr1007/1008; cat. no. #3776; CST), STAT3 (cat. no. #12640; CST) and p-STAT3 (Tyr705; cat. no. #9145; CST) (all 1:1,000).

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Small Interfering RNA, Negative Control

Journal: Oncology Letters

Article Title: Downregulation of amine oxidase copper containing 1 inhibits tumor progression by suppressing IL-6/JAK/STAT3 pathway activation in hepatocellular carcinoma

doi: 10.3892/ol.2021.13118

Figure Lengend Snippet: AOC1-knockdown blocks the IL-6-induced proliferation, migration and invasiveness in HCC cell lines. HCC cell (A) viability, (B) proliferation, (C) migration and (D) invasiveness were analyzed following IL-6 treatment using Cell Counting Kit 8, colony formation, wound-healing and Transwell assays, respectively. *P<0.05 vs. siNC group; # P <0.05 vs. siNC + IL-6 group. AOC1, amine oxidase copper containing 1; HCC, hepatocellular carcinoma; si, small interfering (RNA); NC, negative control.

Article Snippet: The membranes were blocked with 5% fat-free milk at 25°C for 1 h, and then incubated with primary antibodies overnight at 4°C, including AOC1 (cat. no. ab231558; Abcam), E-cadherin [cat. no. #3195; Cell Signaling Technology (CST)], N-cadherin (cat. no. #13116; CST), vimentin (cat. no. #5741; CST), JAK2 (cat. no. #3230; CST), p-JAK2 (Tyr1007/1008; cat. no. #3776; CST), STAT3 (cat. no. #12640; CST) and p-STAT3 (Tyr705; cat. no. #9145; CST) (all 1:1,000).

Techniques: Migration, Cell Counting, Small Interfering RNA, Negative Control